Table S2. Real-time PCR primers and probes for analysis of aire-regulated genes

Gene

Forward primer

Reverse primer

Probe

Chromogranin B

CCAGTGGATAAC AGGGATCACA

GGATAGGGCATT TGAGAGGACTT

AAGAAATGGTGA CTCGCTGCATTATC

Col7A1

ACCCTGTTGCC TCTCATTTCC

TCCATTGTACTC GCAAGGATTG

ACCCTGCCTTCT GATGACACTCC

Prominin 1

AAACCAAACTGT GGATGTCATCAA

CTTTGGCTCATG TCCTTAATGTTG

CGTCAAAAATAC CTTGGACTCCATTAG

SPP1

GATTTGCTTTTG CCTGTTTGG

TGAGCTGCCAGA ATCAGTCACT

ATTGCCTCCTCC CTCCCGGTGA

All probes were conjugated with FAM at the 5′ end and TAMRA quencher at the 3′ end.