Table S1. MALDI-TOF mass spectrometry analysis of the full-length Myo2p globular tail and polypeptides generated by mild trypsin digestion

Polypeptide Predicted MM Experimental MM Difference
  kDa kD D
Recombinant globular tail, 1087–1574 aa 55.194 54.925 269
Globular tail, 1131–1574 aa 50.548 50.503 45
Subdomain I, 1131–1345 aa 24.189 24.191 2
Subdomain II, 1346–1574 aa 26.376 26.399 23

MM, molecular mass.

aCalculated from primary sequences using the ExPASy (Expert Protein Analysis System) proteomics server of the Swiss Institute of Bioinformatics at http://us.expasy.org.