Table S1. MALDI-TOF mass spectrometry analysis of the full-length Myo2p globular tail and polypeptides generated by mild trypsin digestion

Polypeptide Predicted MM Experimental MM Difference
  kDa kD D
Recombinant globular tail, 1087–1574 aa 55.194 54.925 269
Globular tail, 1131–1574 aa 50.548 50.503 45
Subdomain I, 1131–1345 aa 24.189 24.191 2
Subdomain II, 1346–1574 aa 26.376 26.399 23

MM, molecular mass.

aCalculated from primary sequences using the ExPASy (Expert Protein Analysis System) proteomics server of the Swiss Institute of Bioinformatics at